Triplex-forming peptide nucleic acid modified with 2-aminopyridine as a new tool for detection of A-to-I editing.
نویسندگان
چکیده
RNA editing from adenosine to inosine (A-to-I editing) is one of the mechanisms that regulate and diversify the transcriptome. Here, a triplex-forming peptide nucleic acid (PNA) modified with a 2-aminopyridine nucleobase was applied for the recognition of the A-to-I editing event in double-stranded RNAs. The triplex-forming PNA enabled sequence-specific detection of single nucleobase editing at sub-nanomolar concentration.
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ورودعنوان ژورنال:
- Chemical communications
دوره 52 51 شماره
صفحات -
تاریخ انتشار 2016